Device
Part:BBa_K417007:Design
Designed by: Ben Porebski, Andrew Perry Group: iGEM10_Monash_Australia (2010-10-26)
Simple inducible (LacI) ethylene generator
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 912
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Native LacI promoter (BBa_R0010) was chosen since it facilitates easier assembly compared with the shorter alternative BBa_R0011, although it would be worth testing both these promoters. It is unknown if the order of the coding sequences is important in this device.
Source
See part documentation for enzyme coding sequences BBa_K417001 and BBa_K417002 for details on the origin of the sequences.